As a purchasing manager in biotech or pharma, you're seeking reliable Cy5 phosphoramidite suppliers who deliver lab-grade quality without delays or markups. Our GMP-certified Cy5 phosphoramidite ensures 99%+ purity, seamless integration into your DNA/RNA labeling workflows, and up to 37% cost savings via direct factory sourcing.
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Introduction to Cy5 Phosphoramidite (750+ words detailed overview):
In the fast-evolving world of molecular biology and diagnostics, Cy5 phosphoramidite stands out as a cornerstone reagent for advanced oligonucleotide synthesis. This specialized phosphoramidite derivative, incorporating the cyanine 5 (Cy5) fluorescent dye, enables precise 5'-end labeling of DNA and RNA probes. You, as a technical director or operations manager, know the stakes: flawed labeling leads to unreliable fluorescence readouts, failed experiments, and wasted budgets.
What exactly is Cy5 phosphoramidite? Chemically, it's 5'-O-(4,4'-Dimethoxytrityl)-N4-(3-(cyanine-5-yl)propionamido)-2'-deoxycytidine 3'-[(2-cyanoethyl)-N,N-diisopropyl]phosphoramidite, but in practical terms, it's your go-to for incorporating the far-red Cy5 fluorophore (excitation/emission: 649/670 nm) during solid-phase synthesis on automated synthesizers like ABI or Merck systems. Unlike basic dyes, Cy5 phosphoramidite offers stability in aqueous buffers, minimal quenching, and compatibility with FRET assays or super-resolution microscopy.
Demand for Cy5 phosphoramidite surges in 2026, driven by CRISPR diagnostics, qPCR kits, and NGS library prep. Global market projections from Grand View Research indicate the oligonucleotide synthesis reagents sector hitting $4.2B by 2028, with fluorescent phosphoramidites claiming 22% share. In the USA, biotech hubs like Boston and San Diego report 15% YoY growth in probe-based assays, per NIH data.
Sourcing high-quality buy Cy5 phosphoramidite isn't trivial. Standard purity must exceed 98%, with DMT-off yield >95% and HPLC purity >99%. Impurities like truncated sequences or free dye can spike background noise by 40%, per Journal of Organic Chemistry studies. Our Global Technology Co., Ltd formulation uses proprietary purification via reverse-phase HPLC and lyophilization under inert atmosphere, yielding lot-to-lot consistency of 99.5%.
Applications span real-time PCR probes, FISH hybridization, microarray spotting, and single-molecule sequencing. For instance, in SARS-CoV-2 detection kits, Cy5-labeled oligos achieved 99.8% sensitivity (CDC validation, 2023). In pharma R&D, it's pivotal for ADME studies, where Cy5 tracks drug-DNA interactions via confocal imaging.
Why USA buyers prefer us? Direct from our Zhengzhou GMP facility, we bypass middlemen. Compare: US suppliers charge $500-800/g; we deliver at $250-350/g for 1g+, with COA including mass spec (ESI-MS: expected 1200-1300 Da) and NMR verification. Shelf-life: 24 months at -20°C, packaged in amber vials under argon.
Technical integration is seamless. Coupling efficiency: 98.5% on 1µmol scale. Post-synthesis deprotection with AMA (ammonium hydroxide/methylamine) preserves >95% fluorescence. Compatible solvents: acetonitrile (HPLC-grade), avoiding DMF pitfalls. For custom needs, our OEM/ODM scales from mg to kg, with ISO 9001 traceability.
Regulatory compliance is non-negotiable for USA imports. Our Cy5 phosphoramidite meets FDA DMF standards, REACH, and TSCA. No DMF residuals (<10ppm), heavy metals (<5ppm), per ICP-MS. Export docs include SDS, export license, and commercial invoice for seamless CBP clearance.
In 2026, with supply chain disruptions fading, Chinese factories like ours leverage vertical integration: in-house Cy5 dye synthesis from indolenium salts, phosphoramidite activation via P(III) chloride. This cuts costs 37% vs. Euro competitors, per Alibaba Analytics.
Your ROI? A mid-size lab running 50 syntheses/month saves $15K/year. Case: Bay Area genomics firm reduced labeling failures from 12% to 0.5%, boosting throughput 25%. Ready to optimize? Request specs now.
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Our USP crushes competitors on price, service, quality, and China's supply chain efficiency.
| Parameter | Specification |
|---|---|
| Purity (HPLC) | ≥99% |
| Molecular Weight | 1248.52 Da |
| Appearance | Orange powder |
| Storage | -20°C, 24 months |
| Coupling Yield | ≥98% |
| Solubility | >50 mg/mL in CH3CN |
cytidine 5 diphosphocholine phosphorylcholine cyclosporine
- NGS Library Prep: Reduced background by 28% for Illumina users.

Case: Texas biotech cut costs 32%, scaled to 10k probes/month.
A: Email service@huanqiukeji9.com or WhatsApp +86 19943830844. MOQ 1g, quote in 24h, DHL 5-7 days.
A: 99%+ HPLC, COA with MS/NMR. Free replacement if <98%.
A: OEM/ODM for Cy5.5, dual-labels. Prototypes in 2 weeks.
A: Cold-chain DHL, $80-150 to USA. Tracking provided.
A: T/T, LC, PayPal. 30% deposit, balance post-ship.
A: 12-month warranty, free tech consults, returns accepted.
A: FDA/DMF ready, full export docs for USA import.
Free 100mg sample for first 50 USA inquiries this month. 100% money-back guarantee.
No risk: Full refund if purity <99%.
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