Fibronectin Analog CAS NO.125455-58-5

Fibronectin Analog (CAS: 125455-58-5), also known as Fibronectin Fragment III1-C or FN III1-C, is a recombinant or synthetic peptide fragment derived from the first type III domain of fibronectin. This 30-40 amino acid sequence contains the RGD (Arg-Gly-Asp) motif and adjacent synergy sites that mimic the cell-binding domain of native fibronectin. It exhibits high affinity for integrin receptors (particularly α5β1 and αvβ3) and promotes cell adhesion, spreading, and migration. The analog is widely used in cell culture applications, tissue engineering scaffolds, and wound healing research. Supplied as lyophilized powder with ≥95% purity, it is soluble in aqueous buffers and maintains biological activity when coated onto surfaces.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	Fibronectin Analog
CAS NO.	125455-58-5
Appearance	White to off-white lyophilized powder
Molecular Formula	C29H51N11O11
Molecular Weight	729.8 g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water (≥15 mg/mL), dilute PBS, or 0.1% TFA in aqueous solution.
Endotoxin	< 1.0 EU/mg
Biological Activity	Binds to integrin receptors (α₅β₁, αᵥβ₃) to promote cell adhesion and migration; exhibits enhanced stability compared to native fibronectin fragments.
Storage Conditions	Store at -20°C to -80°C. Lyophilized product is stable for 24 months at -20°C; avoid repeated freeze-thaw cycles.
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

The Fibronectin Analog functions primarily through integrin receptor binding via its RGD motif. The RGD sequence (Arg-Gly-Asp) directly interacts with the ligand-binding pocket of integrins α5β1 and αvβ3, triggering intracellular signaling cascades. This engagement leads to focal adhesion complex formation, activation of focal adhesion kinase (FAK), and recruitment of talin and vinculin to adhesion sites. Additionally, the synergy region adjacent to RGD enhances binding affinity and specificity for α5β1 integrin through conformational changes.

2.PRODUCT INDICATIONS

Cell Culture Surface Coating: Enhancing attachment and proliferation of primary cells (endothelial cells, fibroblasts, mesenchymal stem cells) in vitro

Biomaterial Functionalization: Modifying synthetic scaffolds, hydrogels, and medical devices to promote cell integration and tissue regeneration

Integrin Signaling Studies: Investigating integrin-mediated cell adhesion, migration, and mechanotransduction pathways

Wound Healing Assays: Supporting cell migration in scratch wound and transwell migration assays

Stem Cell Differentiation: Promoting osteogenic and chondrogenic differentiation of mesenchymal stem cells on modified surfaces

Angiogenesis Models: Supporting endothelial cell tube formation and vascular network development

3.CLINICAL EFFICACY OF PRODUCT

In vitro studies demonstrate concentration-dependent enhancement of cell adhesion (2-3 fold increase compared to uncoated surfaces) and accelerated wound closure in scratch assays

Animal models of skin wound healing show improved re-epithelialization and collagen deposition when treated with RGD-containing peptide coatings

Bone regeneration studies in rodent defect models indicate enhanced bone formation and mineralization with peptide-functionalized scaffolds

Cardiovascular applications suggest improved endothelialization of vascular grafts coated with fibronectin analogs

4.DELIVERY & SHIPPING

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Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
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Shipping Methods
Main Method: International Air Freight

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