[Nle27]-GRF (1-29) amide (human)

[Nle27]-GRF (1-29) amide (human) is a high-purity synthetic peptide analog of human growth hormone-releasing hormone (GHRH), featuring a norleucine (Nle) substitution at position 27 to enhance metabolic stability and reduce oxidation sensitivity. This 29-amino acid peptide functions as a potent and specific agonist for GHRH receptors, stimulating growth hormone secretion from pituitary somatotrophs with improved in vivo stability compared to native GHRH. With HPLC-verified purity and comprehensive analytical documentation, it provides researchers with a reliable tool for studying growth axis regulation, endocrine physiology, and metabolic processes in both in vitro and in vivo models. The enhanced stability profile makes it particularly valuable for prolonged studies requiring sustained growth hormone stimulation.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	[Nle27]-GRF (1-29) amide (human)
CAS NO.	/
Appearance	White to off-white lyophilized powder
Molecular Formula	C150H247N44O42
Molecular Weight	3338.91g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water, acetic acid (0.1%), and DMSO; may require gentle vortexing for complete dissolution
Endotoxin	< 1.0 EU/mg
Biological Activity	Growth hormone-releasing hormone (GHRH) agonist; stimulates growth hormone secretion from anterior pituitary somatotrophs via activation of GHRH receptor.
Storage Conditions	Store at -20°C or below, protected from light and moisture. After reconstitution, aliquot and store at -20°C.
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

[Nle27]-GRF (1-29) amide (human) binds with high affinity to GHRH receptors on pituitary somatotroph cells, activating G-protein coupled signaling pathways. The norleucine substitution at position 27 enhances peptide stability by reducing methionine oxidation, while maintaining full receptor binding affinity. This binding stimulates adenylate cyclase activity, increasing intracellular cAMP levels and activating protein kinase A (PKA). The downstream effects include phosphorylation of transcription factors (such as CREB), enhanced growth hormone gene expression, and stimulation of growth hormone synthesis and secretion. The C-terminal amidation further enhances receptor binding and peptide stability, resulting in prolonged biological activity compared to native GHRH fragments.

2.PRODUCT INDICATIONS

Endocrine Research: Investigation of growth hormone regulation, pituitary function, and growth axis signaling with enhanced peptide stability

Metabolic Studies: Research on growth hormone's role in metabolism, body composition, and energy homeostasis using stable peptide analogs

Drug Discovery: Screening for GHRH receptor agonists with improved pharmacokinetic properties for therapeutic applications

Receptor Pharmacology: Study of GHRH receptor binding kinetics, signal transduction, and structure-activity relationships

Preclinical Studies: Evaluation of growth-promoting effects and endocrine system modulation in animal models requiring sustained peptide activity

Stability Research: Comparative studies on peptide stability, oxidation resistance, and metabolic half-life

3.CLINICAL EFFICACY OF PRODUCT

Enhanced Stability Profile: Norleucine substitution at position 27 significantly improves metabolic stability and reduces oxidation compared to native GHRH sequences

Validated Bioactivity: Demonstrated potent stimulation of growth hormone secretion in pituitary cells and in vivo models with prolonged activity

High Purity: ≥95% by RP-HPLC with mass spectrometry confirmation of sequence and C-terminal amidation

Improved Pharmacokinetics: Extended half-life and sustained biological effects compared to native GHRH (1-29) fragments

Research Reproducibility: Consistent performance in growth hormone release assays, receptor binding studies, and animal experiments

Quality Assurance: Produced under controlled conditions with low endotoxin levels for research applications

Documentation: Each batch includes Certificate of Analysis with purity, mass spec, and biological activity data

4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.