Casein Kinase II Assay Kit
TECHNICAL SPECIFICATIONS
| Item | Specification |
|---|---|
| Product Name | Casein Kinase II Assay Kit |
| CAS NO. | / |
| Appearance | White to off-white lyophilized powder |
| Molecular Formula | C45H73N19O24 |
| Molecular Weight | 1264.2g/mol |
| Purity (RP-HPLC) | ≥90% |
| Solubility | Assay buffer components are soluble in deionized water; the peptide substrate is soluble in assay buffer or water |
| Endotoxin | ≤1.0 EU/mg |
| Biological Activity | ≥90% specific activity, measured by phosphorylation of the synthetic peptide substrate under standard assay conditions |
| Storage Conditions | Store at -20°C; avoid repeated freeze-thaw cycles. Some components may be stable at 4°C for short-term use. |
| Stock Location | Stock in USA |
| MOQ | 1g |
1.MECHANISM OF ACTION
This kit operates on the principle of measuring the catalytic transfer of a phosphate group from ATP to a CK2-specific substrate. The core reaction utilizes a synthetic peptide substrate (e.g., derived from casein or a consensus sequence like RRRADDSDDDD) that is highly selective for CK2. In the presence of active CK2 enzyme in the sample, the kinase phosphorylates the serine/threonine residues on this substrate.
- Kinase Activity Profiling: Quantifying changes in CK2 activity in response to cellular stimuli (e.g., growth factors, stress), during different cell cycle phases, or in disease states (e.g., cancer, inflammation).
- Drug Discovery & Screening: High-throughput screening (HTS) of compound libraries to identify novel, potent, and selective CK2 inhibitors for therapeutic development in oncology and other diseases.
- Biomarker Research: Measuring CK2 activity as a potential diagnostic or prognostic biomarker in tumor samples or other pathological tissues.
- Purified Enzyme Characterization: Determining the specific activity of recombinant or purified CK2 enzyme, evaluating enzyme kinetics (Km, Vmax), and testing the effects of mutations.
2.PRODUCT INDICATIONS
- Research & Diagnostic Tool Performance: The kit's efficacy is defined by its sensitivity, specificity, reproducibility, and ease of use in accurately measuring CK2 activity in vitro. High-quality kits are essential for generating reliable data.
- Therapeutic Target Validation: CK2 is a validated oncotherapeutic target. It is overexpressed in many cancers (e.g., leukemia, breast, prostate) and promotes cell survival and proliferation. Reliable assay kits are crucial for validating CK2 as a target in preclinical models and for characterizing lead inhibitor compounds.
- Drug Development Enabler: The kit is used extensively in the preclinical pipeline to screen and optimize CK2 inhibitors (e.g., CX-4945/Silmitasertib), which have entered clinical trials for various cancers. The kit's performance directly impacts the efficiency of this discovery process.
- Clinical Correlation: While the kit itself is not a clinical diagnostic, the activity measurements it provides can be correlated with disease stage, patient prognosis, or treatment response in research settings, potentially guiding future diagnostic development.
3.CLINICAL EFFICACY OF PRODUCT
4.DELIVERY & SHIPPING
We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight
We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.
