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Calmodulin-Dependent Protein Kinase II (281 – 289)

Calmodulin-Dependent Protein Kinase II (281-289) is a high-purity synthetic peptide representing the autoinhibitory domain fragment of CaMKII. This 9-amino acid peptide (KRQETDDV) corresponds to residues 281-289 of the human CaMKII α isoform and functions as a competitive inhibitor of CaMKII catalytic activity. Manufactured under stringent quality controls with HPLC-verified purity and comprehensive analytical documentation, this product provides researchers with a specialized tool for studying CaMKII regulation, kinase signaling pathways, and neuronal plasticity mechanisms. The peptide mimics the autoinhibitory interaction that occurs in the inactive state of CaMKII, enabling investigations into calcium/calmodulin-dependent activation, enzyme kinetics, and signal transduction processes in various cellular contexts.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	Calmodulin-Dependent Protein Kinase II (281 - 289)
CAS NO.	/
Appearance	White to off-white lyophilized powder
Molecular Formula	C43H71N15O16S2
Molecular Weight	1118.26 g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water, PBS (pH 7-8), dilute acetic acid
Endotoxin	< 1.0 EU/mg
Biological Activity	The self-inhibition domain fragment of CaMKII can competitively bind to the catalytic domain of CaMKII, simulating a self-inhibition state, and can be used to study the activation/inhibition mechanism, enzyme activity regulation, and signal transduction of CaMKII.
Storage Conditions	Store at -20°C or below, protected from light and moisture. After reconstitution, aliquot and store at -20°C
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

Calmodulin-Dependent Protein Kinase II (281-289) functions as a competitive inhibitor by binding to the catalytic domain of CaMKII, mimicking the autoinhibitory interaction that occurs in the inactive state of the full-length enzyme. In the absence of calcium/calmodulin, this peptide sequence in the intact CaMKII associates with the catalytic site, preventing substrate access and maintaining the kinase in an inactive conformation. The synthetic peptide competes with the endogenous autoinhibitory domain for binding to the catalytic site, thereby inhibiting CaMKII activity. This inhibition is reversible and concentration-dependent, allowing researchers to specifically probe CaMKII function without affecting other kinases. The peptide's mechanism is based on its high-affinity interaction with the catalytic domain, effectively blocking substrate phosphorylation and downstream signaling events.

2.PRODUCT INDICATIONS

Signal Transduction Research: Investigation of CaMKII-dependent signaling pathways in neurons, cardiomyocytes, and other cell types

Enzyme Kinetics: Characterization of CaMKII activation and inhibition mechanisms

Neuronal Plasticity Research: Evaluation of CaMKII's role in synaptic plasticity, LTP, and memory formation

Drug Discovery: Screening for CaMKII modulators and validation of inhibitor specificity

Comparative Studies: Assessment of CaMKII isoform-specific regulation and function

CaMKII Activity Studies: Competitive inhibition assays to measure kinase activity and IC₅₀ determination

3.CLINICAL EFFICACY OF PRODUCT

Biological Activity: Validated as a competitive inhibitor of CaMKII catalytic activity in kinase assays

Sequence Accuracy: Verified 9-amino acid sequence (KRQETDDV) corresponding to CaMKII 281-289

Research Reproducibility: Consistent inhibitory activity across multiple batches and assay formats

Quality Documentation: Certificate of Analysis provided with purity, mass spec, and analytical data

Technical Support: Access to application guidance for kinase inhibition studies and signal transduction research

4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.