HCV NS4A Protein (22-33) (FDA strain)

HCV NS4A Protein (22-33) (FDA Strain) is a synthetic 12-amino acid peptide antigen corresponding to residues 22-33 of the non-structural protein 4A (NS4A) from a reference Hepatitis C Virus (HCV) strain, such as the FDA-standard genotype 1a H77 strain or another relevant reference isolate. NS4A is a multifunctional cofactor essential for viral replication.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	HCV NS4A Protein (22-33) (FDA strain)
CAS NO.	/
Appearance	White to off-white lyophilized powder
Molecular Formula	C54H98N15O14S
Molecular Weight	1213.54g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water, dilute PBS buffer and DMSO (typical solubility ≥1 mg/mL for research use)
Endotoxin	≤1.0 EU/mg
Biological Activity	Immunogenic synthetic peptide fragment of HCV NS4A regulatory protein (FDA strain), critical for HCV NS3/4A protease activation
Storage Conditions	Store at -20°C to -80°C in a sealed, dry container; protect from moisture and avoid repeated freeze-thaw cycles.
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

This peptide functions as a defined linear antigenic determinant. During HCV infection, the NS4A protein is expressed as part of the viral polyprotein and cleaved by the NS3/4A protease. It serves as a cofactor for the NS3 protease and is involved in membrane-associated replication complex formation. As a foreign viral protein, it is processed by the host's immune system, and fragments like the (22-33) region can be presented as epitopes. In research, the synthetic peptide acts as a specific probe:

B-Cell Epitope: It contains linear amino acid sequences that can be recognized by specific antibodies generated during an HCV infection, allowing for the detection of anti-NS4A antibodies in serological assays.
T-Cell Epitope: The sequence may contain motifs that can bind to specific Major Histocompatibility Complex (MHC) molecules, enabling it to stimulate and detect NS4A-specific CD4+ or CD8+ T-cell responses in vitro.
Its mechanism is passive, relying on its specific sequence to interact with immune receptors (antibodies or T-cell receptors).

2.PRODUCT INDICATIONS

Serological Assay Development & Refinement: Used as a coating antigen in enzyme-linked immunosorbent assays (ELISA) or line immunoassays (LIA) to detect and quantify antibodies specifically targeting this NS4A epitope in patient sera. This aids in developing or improving diagnostic tests (e.g., confirmatory immunoblots).
Epitope Mapping & Immune Response Profiling: Mapping the fine specificity of the humoral immune response in HCV-infected or recovered individuals to understand immunodominant regions within NS4A.
T-Cell Immune Monitoring: Stimulating peripheral blood mononuclear cells (PBMCs) in assays like ELISPOT or intracellular cytokine staining (ICS) to measure IFN-γ or other cytokine production from NS4A-specific T cells, correlating responses with disease stage or treatment outcome.

3.CLINICAL EFFICACY OF PRODUCT

Diagnostic Tool Efficacy: The "efficacy" of this peptide is its ability to accurately and specifically detect anti-NS4A antibodies as part of a multi-antigen panel. Anti-NS4A antibodies are a common marker in chronic HCV infection and are included in recombinant immunoblot assays (RIBAs) and modern ELISA/CLIA serological tests to confirm infection and differentiate past exposure from vaccination.
Immune Response Correlation: The presence and pattern of antibodies against NS4A, along with other non-structural proteins (NS3, NS4B, NS5), help distinguish past resolved infection from chronic viremia and are part of the diagnostic algorithm. Research using defined peptides like this helps refine these correlations.
Standardization: The use of a reference strain sequence (e.g., FDA strain) ensures consistency in research and assay calibration, which is critical for developing reliable diagnostic kits with high sensitivity and specificity across different geographical regions with varying HCV genotypes.

4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.