TECHNICAL SPECIFICATIONS
Item
| Specification
|
|---|
Product Name
| Histone H3 (116–136), C116–136
|
CAS NO.
| /
|
Appearance
| White to off-white lyophilized powder
|
Molecular Formula
| C107H195N39O28S
|
Molecular Weight
| 2508.06g/mol |
Purity (RP-HPLC)
| ≥ 95.0% |
Solubility
| Soluble in water, dilute acetic acid, PBS (pH 7‑8) |
| Endotoxin | < 1.0 EU/mg
|
Biological Activity
| Histone H3 C-terminal functional fragment (positions 116-136) contains multiple lysine methylations |
Storage Conditions
| Store at -20°C or below, protected from light and moisture. After reconstitution, aliquot and store at -20°C |
Stock Location
| Stock in USA
|
MOQ
| 1g |
1.MECHANISM OF ACTION
Histone H3 (116–136), C116–136 functions as a biologically active fragment of the core histone H3, which is integral to nucleosome assembly and chromatin architecture. The C-terminal tail of histone H3 extends from the nucleosome core and serves as a platform for post-translational modifications (PTMs) such as methylation, acetylation, and phosphorylation. These PTMs constitute the "histone code" that regulates DNA accessibility, transcription factor recruitment, and gene expression. The specific C-terminal modification (C116–136) may facilitate covalent conjugation to solid supports, tags, or probes, enabling the peptide to be used in affinity purification, co-immunoprecipitation, or detection assays. The peptide can also serve as a substrate for histone-modifying enzymes (e.g., methyltransferases, acetyltransferases) in vitro, allowing the study of enzyme kinetics and inhibitor screening.
2.PRODUCT INDICATIONS
Epigenetics Research: Study of histone modifications, chromatin remodeling, and gene regulation mechanisms.
Enzyme Activity Assays: Substrate for histone-modifying enzymes (HATs, HDACs, HMTs, kinases).
Protein Interaction Studies: Investigation of histone-binding proteins, chromatin readers, and transcriptional regulators.
Antibody Production: Immunogen for generating modification-specific or sequence-specific antibodies.
Drug Discovery: Screening for epigenetic drug candidates targeting histone modifications or chromatin regulators.
Conjugation & Labeling Applications: Use in pull-down assays, affinity chromatography, or diagnostic assay development due to C-terminal modifiable group.
3.CLINICAL EFFICACY OF PRODUCT
Functional Versatility: C-terminal modification enables flexible conjugation to tags, beads, or detection molecules.
Research Reproducibility: Consistent performance in enzymatic assays, binding studies, and immunological applications.
Technical Support: Access to application notes and expert consultation for chromatin and epigenetics research.
4.DELIVERY & SHIPPING
We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight
We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.
