[CysCys21] Atrial Natriuretic Factor (3-28), Rat CAS NO.90984-99-9

[CysCys²¹] Atrial Natriuretic Factor (3‑28), Rat is a synthetic, site-specifically modified analog of rat ANF(3‑28) designed for conjugation and structural studies. This research-grade peptide features a Cys-Cys substitution at position 21, introducing an additional thiol pair while retaining the native disulfide bridge essential for receptor binding. Supplied as a lyophilized powder with ≥95% HPLC purity and low endotoxin levels, it enables site-directed labeling with fluorophores, biotin, or other probes for tracking, pull-down assays, and structural investigations of natriuretic peptide signaling pathways.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	[CysCys21] Atrial Natriuretic Factor (3-28), Rat
CAS NO.	90984-99-9
Appearance	White to off-white lyophilized powder
Molecular Formula	C119H189N43O36S2
Molecular Weight	2862.2g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water, phosphate‑buffered saline (PBS), or dilute acetic acid (e.g., 0.1 % v/v). For best results, first dissolve in a small volume of 0.1 % acetic acid or sterile water to prepare a 0.1‑1.0 mg/mL stock solution, then dilute with assay buffer
Endotoxin	< 1.0 EU/mg
Biological Activity	This analog of rat ANF(3‑28) retains the ability to bind to natriuretic peptide receptors (primarily NPR‑A) and stimulate guanylyl cyclase activity, leading to increased intracellular cGMP levels. The Cys‑Cys substitution at position 21 may be designed to introduce a reactive thiol pair for conjugation, labeling, or structural studies. Bioactivity should be confirmed by cGMP accumulation or vascular relaxation assays.
Storage Conditions	Lyophilized powder: Store desiccated at –20 °C or below, protected from light. Reconstituted solution: Aliquot and store at ≤ –20 °C; avoid repeated freeze‑thaw cycles.
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

The peptide functions as a ligand for natriuretic peptide receptors, primarily NPR‑A, by binding to the extracellular domain through its conserved disulfide-constrained core structure. This interaction activates membrane-bound guanylyl cyclase, increasing intracellular cyclic GMP (cGMP) levels and subsequently activating cGMP-dependent protein kinases. The resulting signaling cascade promotes vasodilation, natriuresis, and diuresis, while inhibiting the renin–angiotensin–aldosterone system. The [CysCys²¹] modification does not disrupt this canonical pathway but provides a reactive handle for covalent conjugation without compromising receptor engagement in appropriate assay systems.

2.PRODUCT INDICATIONS

This modified ANF analog is indicated exclusively for research use in preclinical studies of natriuretic peptide receptor biology and probe development. It is employed as a scaffold for site-specific conjugation to fluorophores, biotin, or other tags to generate labeled ligands for receptor imaging, flow cytometry, immunohistochemistry, and pull-down assays. The unlabeled peptide serves as a cold competitor in displacement studies and can be used in functional assays to assess cGMP accumulation and vascular responses. It is not intended for diagnostic, therapeutic, or clinical applications in humans or animals.

3.CLINICAL EFFICACY OF PRODUCT

As a research reagent and conjugation scaffold, [CysCys²¹] Atrial Natriuretic Factor (3‑28), Rat has no established clinical efficacy for therapeutic use. The product has not undergone clinical development, regulatory approval, or safety evaluation for any medical indication. While it contributes to the development of molecular probes and tools for studying cardiovascular and renal diseases, its utility is confined to laboratory research. There are no proven clinical benefits or safety data supporting its use in human patients.

4.DELIVERY & SHIPPING

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Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
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Shipping Methods
Main Method: International Air Freight

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