Neuropeptide AF (hNPAF), Human

Neuropeptide AF (hNPAF), Human is a 18-amino acid amidated neuropeptide derived from the human precursor, supplied as a lyophilized powder with ≥95% HPLC purity and low endotoxin levels. This research-grade peptide serves as a potent and selective endogenous ligand for the NPFF2 receptor (GPR74), designed for investigating neuropeptide FF/AF signaling pathways in pain modulation, opioid-induced hyperalgesia, cardiovascular regulation, and feeding behavior. It provides a standardized tool for receptor binding assays, functional studies in both in vitroand in vivomodels, facilitating research into the neurobiology of pain and autonomic functions.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	Neuropeptide AF (hNPAF), Human
CAS NO.	/
Appearance	White to off-white lyophilized powder
Molecular Formula	C90H132N26O25
Molecular Weight	1978.21g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water, dilute acetic acid (e.g., 0.1% v/v), or phosphate‑buffered saline (PBS). It is recommended to first dissolve in a small volume of 0.1% acetic acid or sterile water to prepare a 0.1–1.0 mg/mL stock solution.
Endotoxin	< 1.0 EU/mg
Biological Activity	Human Neuropeptide AF (hNPAF) is an endogenous ligand for the NPFF2 receptor (also known as GPR74 or OT7T022). It is involved in modulating pain perception (particularly opioid-induced hyperalgesia), cardiovascular functions, and food intake. Biological activity is typically confirmed by receptor binding assays (competition with radiolabeled NPFF) or functional assays measuring inhibition of adenylate cyclase or calcium mobilization in NPFF2-expressing cells.
Storage Conditions	Lyophilized powder: Store desiccated at –20°C or below, protected from light, for long‑term stability. Reconstituted solution: Aliquot and store at ≤ –20°C; avoid repeated freeze‑thaw cycles. For extended stability, store at –80°C.
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

Neuropeptide AF exerts its biological effects by binding with high affinity and selectivity to the G protein-coupled NPFF2 receptor, primarily coupling to Gi/o proteins to inhibit adenylate cyclase and reduce intracellular cAMP levels. This signaling cascade modulates downstream effectors including potassium channels and calcium mobilization, leading to altered neuronal excitability and neurotransmitter release in central and peripheral nervous systems. The peptide's conserved structure and C-terminal amidation are critical for receptor recognition, resulting in physiological responses such as modulation of opioid-induced hyperalgesia, cardiovascular tone regulation, and appetite suppression through specific neural circuits.

2.PRODUCT INDICATIONS

This hNPAF peptide is indicated exclusively for research applications focused on human neuropeptide FF/AF signaling pathways. It is employed in NPFF2 receptor binding assays, functional studies measuring cAMP inhibition and calcium mobilization, pain perception research (particularly opioid-induced hyperalgesia models), cardiovascular physiology investigations, and feeding behavior studies. The product serves as a reference agonist for validating NPFF2 receptor expression, screening receptor antagonists, and studying the role of NPFF/AF systems in pain management, autonomic regulation, and metabolic disorders. It is not intended for diagnostic, therapeutic, or clinical use in humans or animals.

3.CLINICAL EFFICACY OF PRODUCT

As a research reagent, Neuropeptide AF (hNPAF), Human has no established clinical efficacy or safety profile for therapeutic applications. The peptide has not undergone clinical trials, regulatory approval, or safety evaluation for any medical indication. While it contributes to fundamental understanding of NPFF/AF biology and may inform therapeutic strategies targeting NPFF2 receptors for pain management, opioid tolerance, or cardiovascular disorders, its utility remains confined to laboratory research. Any clinical translation would require development of entirely distinct therapeutic agents with comprehensive preclinical and clinical testing.

4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.