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PAR-4 (1-6) (human) CAS NO.225779-44-2

PAR-4 (1-6) (human) is a synthetic hexapeptide corresponding to the amino terminus of the protease-activated receptor 4 (PAR4) after cleavage at Arg47/Gly48. It serves as a potent and selective agonist of PAR4, effectively activating both wild-type and mutant PAR4 (R47A) at concentrations around 500 μM. This peptide is delivered as a lyophilized powder with high purity (typically >95%) and is designed for use in research settings to study PAR4-mediated signaling pathways, platelet aggregation, and cellular responses.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	PAR-4 (1-6) (human)
CAS NO.	225779-44-2
Appearance	White to off-white lyophilized powder
Molecular Formula	C28H41N7O9
Molecular Weight	619.68g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water or aqueous buffers. For best results, prepare a stock solution in sterile water or PBS and aliquot to avoid repeated freeze-thaw cycles.
Endotoxin	< 1.0 EU/mg (determined by LAL test)
Biological Activity	Exhibits biological activity consistent with the PTH (64-84) fragment. Specific activity may vary depending on the assay system and experimental conditions.
Storage Conditions	Store lyophilized powder at -20°C or below. For long-term storage, keep at -80°C. Protect from light and moisture. Reconstituted solutions should be stored at -20°C or -80°C in single-use aliquots.
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

PAR-4 (1-6) acts by mimicking the endogenous tethered ligand sequence of human PAR4, specifically targeting residues 1–6 (Gly-Tyr-Pro-Gly-Gln-Val). Upon binding, it triggers intracellular calcium mobilization and activates protein kinase C (PKC) pathways, leading to platelet aggregation. Unlike thrombin, which requires specific cleavage sites, this peptide can activate PAR4 independently of proteolytic cleavage, including mutant receptors like PAR4 R47A, making it a valuable tool for dissecting receptor-specific signaling without confounding protease effects.

2.PRODUCT INDICATIONS

This peptide is primarily indicated for use in in vitro research to probe PAR4 function, particularly in platelet aggregation studies and receptor activation assays. It is also utilized in vascular smooth muscle cell (SMC) research to investigate mitogenic responses and ERK1/2 phosphorylation. Due to its specificity for PAR4, it is employed to distinguish PAR4-mediated effects from those of other PAR subtypes (e.g., PAR1) in complex biological systems.

3.CLINICAL EFFICACY OF PRODUCT

While PAR-4 (1-6) itself is not a therapeutic agent, its role in elucidating PAR4 biology has significant clinical implications. Studies using this agonist have demonstrated its ability to induce sustained platelet aggregation and promote vascular smooth muscle cell proliferation. These findings support the exploration of PAR4 as a therapeutic target, with emerging clinical strategies focusing on PAR4 antagonists (e.g., BMS-986120) for anti-thrombotic applications, leveraging insights gained from agonist studies like those involving PAR-4 (1-6).

4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
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Shipping Methods
Main Method: International Air Freight

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