AKT/PKB/Rac – Protein Kinase Substrate CAS NO.324029-01-8

AKT/PKB/Rac – Protein Kinase Substrate (commonly known as AKTide-2T) is a high-purity synthetic peptide extensively used in cell signaling and kinase biology research. It is engineered to serve as a highly specific and sensitive substrate for AKT (also known as PKB or Rac) serine/threonine kinases, allowing researchers to accurately measure kinase activity in vitro. This peptide is an essential tool for scientists studying the PI3K/AKT/mTOR signaling pathway, which is pivotal in regulating cell growth, survival, metabolism, and apoptosis. Its consistent quality and defined phosphorylation characteristics make it ideal for academic laboratories, pharmaceutical R&D, and biotechnology companies engaged in kinase drug discovery, assay development, and signal transduction studies.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	AKT/PKB/Rac – Protein Kinase Substrate (AKTide-2T)
CAS NO.	324029-01-8
Appearance	White to off-white lyophilized powder
Molecular Formula	C74H114N28O20
Molecular Weight	~1715.91 g/mol
Purity (RP-HPLC)	≥98%
Solubility	Soluble in water, DMSO; insoluble in ethanol, acetone, chloroform
Endotoxin	≤10 EU/mg
Biological Activity	Specific substrate for AKT/PKB/Rac kinases (phosphorylation at Ser, Km = 3.9 µM); competitively inhibits histone H2B phosphorylation by AKT (Ki = 12 µM)
Storage Conditions	Store at -20°C, protected from light and moisture
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

This peptide functions by containing a conserved optimal phosphorylation motif recognized by AKT/PKB/Rac kinases, wherein the serine residue within its sequence is specifically phosphorylated by the active kinase in an ATP-dependent manner. Beyond serving as a phosphate acceptor, it acts as a competitive inhibitor against natural substrates like histone H2B, binding to the kinase's active site with high affinity to block endogenous substrate phosphorylation. This dual capability allows researchers to utilize the peptide both to quantify the enzymatic rate of AKT (via radiolabeled or fluorescent ATP consumption assays) and to probe the substrate specificity and inhibition constants of potential kinase inhibitors in biochemical screening platforms.

2.PRODUCT INDICATIONS

The product is indicated for laboratory research applications focusing on oncology, diabetes, immunology, and neuroscience, particularly where the PI3K/AKT pathway is a central regulatory mechanism. It is suitable for use in kinase activity assays, substrate specificity profiling, inhibitor screening campaigns, and mechanistic studies of AKT signaling in cellular lysates or purified systems. Researchers and technicians in drug discovery units utilize this peptide to validate hit compounds from high-throughput screenings, optimize assay conditions for preclinical studies, and characterize the kinetic parameters of novel AKT modulators, ensuring robust and reproducible data generation in non-clinical settings.

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3.CLINICAL EFFICACY OF PRODUCT

As a specialized research reagent, this peptide is not administered to humans or animals therapeutically, yet its "efficacy" is demonstrated by its critical role in validating the preclinical efficacy of numerous AKT-targeted therapies currently in clinical trials. It provides a reliable and standardized readout for measuring how effectively a candidate drug can inhibit AKT kinase activity in vitro, thereby predicting its potential to disrupt pathological signaling in cancer and metabolic disorders. The peptide’s well-characterized kinetic values (Km and Ki) ensure that experimental results are quantitatively comparable across different studies, significantly de-risking the early-stage translation of kinase inhibitor leads into viable clinical candidates.

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4.Certificate

GlobalTech Co., Ltd. is committed to providing high-purity biotechnological peptide products, adhering strictly to quality standards and obtaining internationally recognized certifications.

Our production partners all employ GMP-compliant production systems and possess standardized quality control processes covering raw material procurement, synthesis, purification, testing, packaging, and storage. Each batch of product comes with complete documentation, including a Certificate of Analysis (COA), HPLC purity report, mass spectrometry (MS) chromatograms, and third-party testing reports available upon request.

We prioritize:

1.A rigorous quality management system

2.Batch traceability and stability data

3.Professional laboratory testing and validation

4.Compliance with international trade standards

Our commitment to quality, transparency, and regulatory compliance ensures the provision of reliable, stable, and research-grade peptide products to our global partners.

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5.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.

Professional and Secure Packaging

Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.

We can also customize packaging according to customer needs.

Shipping Methods

Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.

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Global Technology Co., Ltd. is a modern and advanced enterprise specializing in the production and export of active pharmaceutical ingredients (APIs), peptides, and plant extracts. The company invests in and collaborates closely with domestic manufacturers holding GMP, DMF, and FDA certifications, as well as laboratories at renowned universities, to meet diverse customer needs ranging from gram-level to ton-level. This enables Dejurui Pharmaceutical to provide customers with more flexible and convenient access to cost-effective, high-quality products and superior services.

We focus on the research and development and supply of high-quality chemical products, strictly adhering to international standards and quality systems. Our products are stable in performance and reliable in purity, widely used in industrial manufacturing, scientific research, and the fine chemical industry. We provide solid support at every stage with a professional and responsible attitude.

Hot Tags: AKT Substrate, AKTide-2T, Kinase Assay, PI3K/AKT/mTOR, Signal Transduction, Phosphorylation, Peptide Reagent, Cell Signaling, Cancer Research, Drug Discovery, Serine/Threonine Kinase, Biochemistry, Research Peptide, High Purity, In Vitro Assay

If you have any product requirements, please send an email to:business@huanqiukeji9.com;whatsapp:85270846510