Histone H3 (116–136), N15–39

Histone H3 (116–136), N15–39 is a synthetic 21-amino acid peptide fragment derived from the C-terminal tail of Histone H3. This specific sequence corresponds to a region containing key lysine and arginine residues (including K122, K128, K133, and K136) that are critical sites for various post-translational modifications (PTMs), particularly methylation.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	Histone H3 (116–136), N15–39
CAS NO.	/
Appearance	White to off-white lyophilized powder
Molecular Formula	C112H197N39O30
Molecular Weight	2570.06g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water, dilute Tris-HCl (pH 7.5-8.0), 0.1% TFA aqueous solution; moderately soluble in DMSO
Endotoxin	< 1.0 EU/mg
Biological Activity	Site-specific modified 21-mer fragment of human Histone H3 (116-136) with N15-39 modification.
Storage Conditions	Store at -20°C to -80°C in a sealed, dry and light-protected container; avoid repeated freeze-thaw cycles
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

This peptide does not have an intrinsic biological activity in isolation. Its function is defined by its role as a specific substrate or binding target for chromatin-associated proteins and enzymes. As part of the native histone H3 tail in vivo, this region protrudes from the nucleosome and is a hotspot for dynamic PTMs that regulate chromatin structure and gene expression.

2.PRODUCT INDICATIONS

Enzyme Activity Assays: Serving as a substrate in in vitrohistone methyltransferase (HMT) or demethylase (KDM) activity assays, often using radioactive (³H-SAM) or antibody-based detection to measure modification levels.
Epitope Mapping & Antibody Validation: Used as a critical control antigen to test the specificity of commercial or custom antibodies against H3 modifications (methylation, acetylation) within this sequence via dot blot, peptide array, or competitive binding assays.
Interaction Studies: Employed in pull-down assays, isothermal titration calorimetry (ITC), or surface plasmon resonance (SPR) to study the binding affinity and specificity of chromatin "reader" proteins for specific modification states on this H3 tail region.
Chromatin Biochemistry: Investigating the biochemical consequences of specific modifications in this region on nucleosome stability, higher-order chromatin folding, or recruitment of effector complexes in reconstituted systems.

3.CLINICAL EFFICACY OF PRODUCT

Research Tool Efficacy: Its efficacy is defined by its performance as a pure, well-defined substrate that enables precise measurement of enzyme activity and antibody specificity, generating reliable and reproducible epigenetic data.
Disease Relevance & Target Validation: The specific modifications on this H3 region (e.g., H3K122me, H3K128me) are implicated in gene regulation, DNA repair, and cancer. Aberrant activity of the enzymes that modify these sites (e.g., NSD2/MMSET in multiple myeloma) are validated therapeutic targets.
Drug Discovery Enabler: This peptide is used in high-throughput screening (HTS) campaigns to identify and characterize small-molecule inhibitors of histone methyltransferases or demethylases that are drivers of cancers and other diseases.
Diagnostic Development: Antibodies validated using this peptide are essential reagents for diagnostic assays that detect specific histone modification patterns in tumor samples (e.g., by immunohistochemistry), which can have prognostic value.
Clinical Connection: While the peptide is not a drug, the enzymes and modifications it helps study are targeted by drugs in development (e.g., EZH2 inhibitors, LSD1 inhibitors). This tool is foundational to that preclinical research.

4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.