Histone H3 (1 – 25),amide

Histone H3 (1-25), amide is a high-purity synthetic peptide representing the N-terminal 25 amino acids of human Histone H3, with C-terminal amidation for enhanced stability. This peptide includes key lysine residues (K4, K9, K14, K18, K23) that are major sites for post-translational modifications (PTMs) such as acetylation, methylation, and phosphorylation, which are critical for chromatin structure and gene expression regulation. Manufactured under stringent quality controls with HPLC-verified purity and comprehensive analytical documentation, this peptide provides researchers with a precise tool for studying epigenetic mechanisms, histone-modifying enzyme activity, and chromatin biology.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	Histone H3 (1 – 25),amide
CAS NO.	/
Appearance	White to off-white lyophilized powder
Molecular Formula	C110H202N42O32
Molecular Weight	2625.1g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water, dilute acetic acid, PBS (pH 7‑8)
Endotoxin	< 1.0 EU/mg
Biological Activity	The N-terminal segment (positions 1-25) of histone H3 is C-terminusally amidated. This region contains multiple lysine residues and can be modified by acetylation, methylation, and other processes, participating in the regulation of chromatin structure and gene expression. It is commonly used in epigenetic modification, antibody production, and protein-protein interaction studies.
Storage Conditions	Store at -20°C or below, protected from light and moisture. After reconstitution, aliquot and store at -20°C
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

Histone H3 (1-25), amide functions as a biologically active fragment of the core histone H3, which is integral to nucleosome structure and chromatin organization. The N-terminal tail of histone H3 protrudes from the nucleosome and serves as a platform for dynamic post-translational modifications (PTMs). These PTMs—such as acetylation, methylation, and phosphorylation—create a "histone code" that regulates DNA accessibility, transcription factor recruitment, and gene expression. Specifically, this peptide can be used as a substrate for histone acetyltransferases (HATs), methyltransferases, kinases, and deacetylases in vitro assays. It also serves as an antigen for generating antibodies targeting specific histone modifications, enabling detection and quantification of epigenetic marks in cellular and clinical samples.

2.PRODUCT INDICATIONS

Epigenetic Research: Study of histone modifications, chromatin remodeling, and gene expression regulation

Enzyme Activity Assays: Substrate for histone-modifying enzymes (HATs, HDACs, HMTs, kinases)

Antibody Production: Immunogen for generating site- and modification-specific antibodies

Drug Discovery: Screening for epigenetic drug candidates targeting histone modifications

Chromatin Biology: Investigation of nucleosome dynamics and protein-histone interactions

Diagnostic Development: Epigenetic biomarker discovery and assay development

3.CLINICAL EFFICACY OF PRODUCT

Functional Integrity: Validated as a substrate for key histone-modifying enzymes (e.g., HATs, HDACs)

Reproducible Performance: Consistent results in enzymatic, binding, and immunological assays

Low Endotoxin:<1.0 eu="">

Technical Support: Application guidance for epigenetic and chromatin research

4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.