Caspase 3 Substrate, Colorimetric

Caspase-3 Substrate (Colorimetric) is a synthetic peptide conjugate specifically designed to detect and quantify the activity of caspase-3, a key executioner cysteine protease in the apoptotic (programmed cell death) pathway. This ready-to-use research-grade reagent provides a simple, colorimetric readout, enabling convenient and direct measurement of apoptosis in cell lysates and enzyme preparations without the need for specialized equipment.

Send Email

Download

TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	Caspase 3 Substrate, Colorimetric
CAS NO.	/
Appearance	White to off-white lyophilized powder
Molecular Formula	C₂₃H₂₈N₆O₁₁S
Molecular Weight	596.56 g/mol
Purity (RP-HPLC)	≥98%
Solubility	Soluble in DMSO (≥20 mg/mL) or DMF; sparingly soluble in aqueous buffers
Endotoxin	≤1.0 EU/mg
Biological Activity	Chromogenic substrate for Caspase-3; releases p-nitroaniline (pNA) upon cleavage, producing a yellow color detectable at 405 nm.
Storage Conditions	Store at -20°C in a desiccated, light-protected environment; avoid repeated freeze-thaw cycles.
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

This substrate operates on a protease cleavage-release principle. It consists of a short peptide sequence (commonly DEVD) that mimics the native cleavage site of caspase-3, linked to a chromogenic reporter molecule, most commonly para-nitroaniline (pNA). During apoptosis, active caspase-3 specifically recognizes and cleaves the peptide bond after the aspartic acid (D) residue in the DEVD sequence. This cleavage liberates the free pNA chromophore, which absorbs light strongly at a wavelength of 405-410 nm, producing a yellow color. The rate of increase in absorbance is directly proportional to the amount of active caspase-3 in the sample.

2.PRODUCT INDICATIONS

This product is indicated for a wide range of apoptosis research in cell biology, oncology, toxicology, and drug discovery. Key applications include: measuring caspase-3 activation as a definitive biochemical marker of apoptosis in treated cell cultures; screening for pro-apoptotic or anti-apoptotic compounds in drug development; studying the mechanisms of cell death induced by chemotherapeutic agents, toxins, or other stimuli; and comparing apoptosis levels across different experimental conditions in a simple, plate-based assay format.

3.CLINICAL EFFICACY OF PRODUCT

The Caspase-3 Substrate itself is a research tool, not a therapeutic. Its clinical and translational efficacy lies in its vital role as a standardized biomarker assay. It is used extensively in: 1) Preclinical Drug Development: To evaluate the efficacy of novel anti-cancer drugs by confirming they induce apoptosis in target cells. 2) Toxicology Studies: To assess the apoptotic potential of new chemical entities. 3) Basic Cancer Research: To understand dysregulated apoptosis in tumors and resistance mechanisms. The data generated using this substrate directly informs the progression of potential therapies into clinical trials and helps elucidate disease mechanisms.

4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.