Calcineurin Substrate CAS NO.113873-67-9

Calcineurin Substrate (CAS NO. 113873-67-9) is a synthetic phosphopeptide designed as a specific and sensitive substrate for the Ca²⁺/calmodulin-dependent serine/threonine protein phosphatase, calcineurin (also known as Protein Phosphatase 2B, PP2B). This research-grade peptide contains a phosphorylated serine or threonine residue within a sequence specifically recognized and dephosphorylated by calcineurin, making it an essential tool for enzymatic activity assays and signal transduction research.

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	Calcineurin Substrate
CAS NO.	113873-67-9
Appearance	White to off-white lyophilized powder
Molecular Formula	C₅₄H₈₉N₁₅O₁₆
Molecular Weight	1208.37 g /mol
Purity (RP-HPLC)	≥98%
Solubility	Soluble in water, dilute acetic acid, or DMSO
Endotoxin	≤1.0 EU/mg
Biological Activity	A highly specific peptide substrate for calcineurin (protein phosphatase 2B); used to quantify calcineurin activity in enzymatic assays by monitoring dephosphorylation of its arginine-rich motif.
Storage Conditions	Store at -20°C to -80°C in a dry environment. Lyophilized powder is stable for 24 months at -20°C. Reconstituted solutions should be stored at -20°C and used within 3 months, avoiding repeated freeze-thaw cycles.
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

This peptide functions as a direct biochemical substrate. It contains a phospho-serine/threonine residue that mimics a natural phosphorylation site on calcineurin's physiological targets (e.g., NFAT, DARPP-32). In the presence of active calcineurin, calcium, and calmodulin, the enzyme catalyzes the hydrolysis of the phosphate group from the peptide. This dephosphorylation reaction can be quantitatively measured using various detection methods (e.g., malachite green for free phosphate release, fluorescence, or absorbance change with coupled enzyme systems), providing a direct readout of calcineurin phosphatase activity.

2.PRODUCT INDICATIONS

This product is indicated for fundamental biochemical and pharmacological research involving calcium signaling and protein dephosphorylation. Key applications include: quantitatively measuring calcineurin enzymatic activity in cell lysates, tissue extracts, or purified enzyme preparations; screening and characterizing inhibitors of calcineurin (e.g., cyclosporin A, FK506/tacrolimus) for drug discovery and immunology research; studying the regulation of calcineurin activity by Ca²⁺/calmodulin and other modulators; and as a standard tool in research on T-cell activation, neuronal signaling, and cardiac hypertrophy where calcineurin-NFAT signaling is pivotal.

3.CLINICAL EFFICACY OF PRODUCT

The Calcineurin Substrate peptide itself is not a therapeutic but a critical diagnostic and research reagent. Its "efficacy" is defined by its role in enabling the accurate measurement of calcineurin activity. This is directly applied in: 1) Therapeutic Drug Monitoring (TDM): Assays using this substrate are fundamental for monitoring the pharmacodynamic effect of calcineurin inhibitors (CNIs) like tacrolimus and cyclosporin in transplant patients, ensuring optimal immunosuppression. 2) Drug Discovery: It is indispensable for screening and developing new immunosuppressive agents. 3) Disease Research: It aids in studying pathological calcineurin signaling in cardiac, neurological, and autoimmune disorders.

4.DELIVERY & SHIPPING

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