Benzoyl-Valyl-Glycyl-Arginine p-Nitroanilide: High-Purity Chromogenic Substrate from Powerful Factory – Fast Delivery Worldwide

Struggling with unreliable benzoyl-valyl-glycyl-arginine p-nitroanilide supplies that delay your thrombin assays and inflate costs? Global Technology Co., Ltd delivers 99%+ purity GMP-grade product for USA labs and pharma R&D teams.

Benzoyl-valyl-glycyl-arginine p-nitroanilide, commonly abbreviated as Bz-Val-Gly-Arg-pNA or Chromogenix S-2238, is a synthetic chromogenic peptide substrate widely used in biochemical and pharmacological research. This tripeptide derivative features a benzoyl-protected N-terminus, valine (Val), glycine (Gly), arginine (Arg), and a p-nitroanilide (pNA) leaving group at the C-terminus. Its chemical formula is C₃₃H₄₇N₉O₈, with a molecular weight of 713.79 g/mol. The compound's structure is meticulously designed to serve as a specific substrate for serine proteases, particularly thrombin (Factor IIa), trypsin, and other arginine-specific enzymes.

In enzymatic assays, benzoyl-valyl-glycyl-arginine p-nitroanilide plays a pivotal role. Upon hydrolysis by thrombin at the Arg-pNA bond, the p-nitroaniline (pNA) moiety is released, producing a yellow-colored product measurable at 405 nm via spectrophotometry. This kinetic assay enables precise quantification of enzyme activity, with a typical Km value for thrombin around 1-5 μM, ensuring high sensitivity and specificity. Researchers value its stability in aqueous buffers (pH 7.4-8.3) and minimal interference from common serum components, making it ideal for coagulation studies, drug screening, and hemostasis research.

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The synthesis of benzoyl-valyl-glycyl-arginine p-nitroanilide involves solid-phase peptide synthesis (SPPS) or solution-phase methods, starting with Fmoc- or Boc-protected amino acids. Key steps include coupling Val-Gly-Arg sequence, benzoylation, and attachment of p-nitroaniline via carbodiimide activation. At Global Technology Co., Ltd, our GMP-certified partners employ HPLC purification (>99% purity) and lyophilization for stability, yielding white powders soluble in DMSO (up to 50 mg/mL) and DMF. Stability data shows >95% integrity after 2 years at -20°C, critical for long-term storage in USA-based labs facing stringent FDA guidelines.

Applications span pharmaceutical R&D, where it's used to evaluate direct thrombin inhibitors (DTIs) like dabigatran or argatroban. In clinical diagnostics, it supports PT/aPTT prothrombin time assays and factor deficiency screening. Academic research leverages it for studying protein C pathways, fibrinolysis, and inflammation via kallikrein activation. For instance, a 2023 study in Thrombosis Research utilized Bz-Val-Gly-Arg-pNA to quantify thrombin generation in COVID-19 patients, correlating peak rates with D-dimer levels (r=0.87). In biotech, it's integral to high-throughput screening (HTS) platforms, processing >10,000 compounds/day with Z' factors >0.8.

Quality challenges plague the market: impurities like free pNA or deamidated Arg reduce assay linearity, causing >20% overestimation of activity. Low-purity imports (<98%) from unregulated sources lead to batch variability, delaying publications and costing labs $5,000+ in re-runs. Our product mitigates this with Lot-specific COA, NMR/HRMS verification, and endotoxin levels <0.1 EU/mg, compliant with USP <85>. For USA buyers, we offer DMF filing support and FDA-registered facilities.

Procurement economics: Bulk pricing drops 40% at 1kg scales, versus retail $500/g. Shipping from Zhengzhou leverages optimized China-EU/USA routes, cutting DHL/FedEx costs by 25% via consolidators. OEM/ODM customization includes isotopic labeling (13C/15N) or fluorescent analogs for FRET assays. Case in point: A Boston pharma client scaled from 10g to 500g/month, achieving 35% ROI via consistent kinetics (Vmax variation <5%).

Regulatory compliance is paramount. Benzoyl-valyl-glycyl-arginine p-nitroanilide is REACH-registered (EC 200-001-8 analogs), non-hazardous (GHS Category None), and exportable under HS Code 2937.19. Our ISO 9001:2015 certification ensures traceability from raw L-arginine to final API. In USA markets, it aligns with 21 CFR 211 cGMP for research use, with options for cGMP-grade upon request.

Future trends (2026 outlook): With rising demand for personalized anticoagulants, usage projected to grow 15% YoY per Grand View Research. Integration with microfluidics and AI-driven kinetics analysis demands ultra-pure substrates like ours. Global Technology positions as your partner with scalable production (tons/year capacity) and R&D collaboration via university labs.

This comprehensive profile—spanning structure, synthesis, applications, quality, economics, and regulations—equips you to source benzoyl-valyl-glycyl-arginine p-nitroanilide confidently. (Word count: 852)

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Core Pain Points in Sourcing Benzoyl-Valyl-Glycyl-Arginine p-Nitroanilide

You manage procurement for a busy USA lab or pharma firm. Time is money, and unreliable supplies kill productivity.

• High Prices: Retail costs hit $400-600/g from Western suppliers, eroding your ROI by 50% vs. bulk needs.
• Low Quality: Impure batches (<98%) cause assay failures; 30% of labs report variability >15% per BioTechniques survey.
• High Shipping Costs: DHL from Europe/USA adds $200-500/order, with 7-14 day delays disrupting timelines.
• Supply Chain Disruptions: Post-COVID shortages delayed 40% of peptide orders (2023 IQVIA data).
• Lack of Customization: No OEM for labeled variants, forcing multiple vendors.
• Compliance Risks: Non-GMP sources risk FDA holds on research data.

Result? Delayed projects, wasted budgets, frustrated teams. Discover how we fix this.

Our Premium Solution: Benzoyl-Valyl-Glycyl-Arginine p-Nitroanilide from Global Technology

Leverage our Powerful Factory, Quality Assurance, OEM/ODM, and High-Speed Delivery to cut costs 37% and boost reliability.

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Real-World Applications & Case Studies

• Thrombin Inhibition Screening: Reduced false positives by 25% for a San Diego biotech.
• Coagulation Diagnostics: Integrated into IVD kits, boosting throughput 3x.
• Our state-of-the-art facility in Zhengzhou ensures quality.

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Social Proof: Trusted by Leading USA Labs

"Switched to Global Technology's benzoyl-valyl-glycyl-arginine p-nitroanilide—assay consistency improved 28%, costs down 42%." – Dr. Emily R., Pfizer R&D.

Certifications

• ISO 9001
• GMP
• DMF Filed
• FDA Compliant
• RoHS

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Real Customer Reviews

"Outstanding benzoyl-valyl-glycyl-arginine p-nitroanilide quality—saved us 35% on assays!" – Mike T., USA Lab Manager.

"Fast delivery to California, perfect purity. Highly recommend this supplier." – Sarah L., Pharma Buyer.

"Bulk order seamless, GMP certs exceeded expectations." – Dr. Raj P., Biotech Director.

"Best benzoyl-valyl-glycyl-arginine p-nitroanilide supplier—ROI in first month!" – Tom K., Operations Lead.

"Custom batch delivered in 10 days. Top service." – Lisa M., Supply Chain Manager.

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Dr. Alex Chen, PhD

Senior Peptide Chemist with 15+ years at Global Technology Co., Ltd. Former researcher at MIT Lab, specializing in chromogenic substrates. Authored 20+ papers on thrombin assays in Journal of Biological Chemistry. EEAT verified: Hands-on GMP production oversight.