Biotin – Gastrin Releasing Peptide, human
TECHNICAL SPECIFICATIONS
| Item | Specification |
|---|---|
| Product Name | Biotin-Gastrin Releasing Peptide, Human (Biotin-hGRP) |
| CAS NO. | 133062-24-7 |
| Appearance | White to off-white lyophilized powder |
| Molecular Formula | C140H218N40O33S3 |
| Molecular Weight | 3085.74 g/mol |
| Purity (RP-HPLC) | ≥ 95.0% |
| Solubility | Soluble in water (≥ 5 mg/mL), dilute acetic acid, and DMSO; forms a clear solution at 1 mg/mL in water. |
| Endotoxin | < 1.0 EU/mg |
| Biological Activity | Retains native hGRP activity: stimulates gastrin release and acts as a mitogenBiotin label enables detection via streptavidin-based assays |
| Storage Conditions | Store at -20°C to -80°C in a desiccated environmentAvoid repeated freeze-thaw cycles; aliquot for long-term storage |
| Stock Location | Stock in USA |
| MOQ | 1g |
1.MECHANISM OF ACTION
Biotin-GRP functions identically to native human GRP by specifically binding and activating the Gastrin-Releasing Peptide Receptor (GRPR or BB2 receptor), a G-protein coupled receptor of the Gq/11 subtype. Upon binding, it triggers the canonical GRP signaling cascade: activation of phospholipase C (PLC), generation of inositol trisphosphate (IP₃) and diacylglycerol (DAG), leading to intracellular calcium mobilization and protein kinase C (PKC) activation.
- GRPR Detection & Localization: Fluorescent or enzymatic immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to visualize GRPR expression and distribution in cells, tissues, or tumor samples.Affinity Pull-Down & Proteomics: Isolating GRP-bound receptors or associated protein complexes from cell lysates using streptavidin-coupled beads for mass spectrometry or Western blot analysis.Biosensor & Assay Development: Immobilization on streptavidin-coated surfaces (e.g., SPR chips, ELISA plates) for binding kinetics studies, receptor-ligand interaction analysis, or high-throughput drug screening.Receptor Trafficking Studies: Tracking the internalization and intracellular fate of the GRP-GRPR complex in real-time using fluorescent streptavidin conjugates.
- Research Utility: Its value lies in enabling critical preclinical research that informs clinical translation. It is extensively used to characterize GRPR expression profiles in human cancers (e.g., prostate, breast, lung), which is a prerequisite for developing targeted therapies and diagnostics.
- Diagnostic Development: The biotin-streptavidin system is a cornerstone in vitrodiagnostic technology. Biotin-GRP can be utilized in the development and calibration of research-use-only (RUO) or prototype in vitrodiagnostic assays to detect GRPR levels in patient samples.
- Therapeutic Insights: Research using this tool helps validate GRPR as a target, screen for antagonists, and understand signaling pathways, indirectly contributing to the development of GRPR-targeted radioligands and therapeutics currently in clinical trials.
- No Therapeutic Use: This conjugate is not administered to humans. Clinical efficacy data pertains to the GRP/GRPR pathway itself, not to this detection reagent.
2.PRODUCT INDICATIONS
3.CLINICAL EFFICACY OF PRODUCT
4.DELIVERY & SHIPPING
We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight
We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.
