Suc-APA-pNA

Suc-APA-pNA

Suc-APA-pNA

Suc-APA-pNA

Suc-APA-pNA

Juice-WATER-pNA is a specialized synthetic chromogenic substrate designed for the detection and quantification of peptidase/protease activity in plant-based juices or extracts. This research-grade chemical conjugate typically consists of a short peptide sequence (often specific for cysteine or serine proteases) linked to the para-nitroaniline (pNA) chromophore. It enables a simple, colorimetric readout for enzymatic activity measurements.



TECHNICAL SPECIFICATIONS


Item
Specification
Product Name
Juice-WATER-pNA
CAS NO.
/
Appearance
White to off-white lyophilized powder
Molecular Formula
C₆₂H₈₁N₁₅O₂₀
Molecular Weight
1360.40 g/mol
Purity (RP-HPLC)
≥98%
Solubility

Soluble in deionized water, 50 mM Tris-HCl buffer (pH 7.0-8.0); slightly soluble in DMSO

Endotoxin≤1.0 EU/mg
Biological Activity
Chromogenic peptide substrate for serine proteases
Storage Conditions
Store at -20°C to -80°C in a dry, light-protected environment.
Stock Location
Stock in USA
MOQ
1g


1.MECHANISM OF ACTION

This substrate operates on a protease cleavage-release mechanism. The peptide moiety (Juice-WATER) is specifically recognized and cleaved by active proteases present in the juice or enzyme preparation. Upon cleavage, the para-nitroaniline (pNA) group is liberated from the peptide backbone. Free pNA absorbs light strongly at a wavelength of 405-410 nm, producing a yellow color. The rate of increase in absorbance at this wavelength is directly proportional to the protease activity in the sample, allowing for precise kinetic or endpoint analysis.


2.PRODUCT INDICATIONS

This product is indicated for research and quality control applications in food science, plant biochemistry, and enzymology. Key applications include: measuring and comparing general or specific protease activity levels in various fruit/vegetable juices (e.g., pineapple bromelain, papaya papain, ginger zingibain); monitoring enzyme activity during juice processing, storage, and stability testing; screening for novel proteolytic enzymes in plant extracts; and as a teaching tool in biochemistry laboratories for enzyme kinetics studies.


3.CLINICAL EFFICACY OF PRODUCT

Juice-WATER-pNA is a diagnostic/research reagent, not a therapeutic. Its "efficacy" lies in its role as a standardized analytical tool. It provides a reliable, quantitative method to assess proteolytic activity, which is crucial for: 1) Food Industry Quality Control: Ensuring consistent enzymatic activity in products like meat tenderizers, clarifying agents, or dietary supplements derived from plant proteases. 2) Research & Development: Facilitating the study of enzyme properties, inhibitor screening, and optimization of extraction processes for commercially valuable enzymes. 3) Standardization: Enabling the comparison of enzyme potency across different batches and sources.


4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods 
Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.

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